Able AI
What's the best dilution for BL21(DE3)-lysate in ?
Tested Applications
| Positive WB detected in | Total bacteria lysates of BL21(DE3) |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10558-1-Ig targets BL21(DE3)-lysate in WB, ELISA applications and shows reactivity with bacteria samples.
| Tested Reactivity | bacteria |
| Cited Reactivity | zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0048 Product name: Recombinant BL21(DE3)-lysate protein Source: e coli.-derived, Unknow Tag: N/A Sequence: Predict reactive species |
| Full Name | BL21(DE3)-lysate |
| Calculated Molecular Weight | 1-300 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_1607762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The BL21(DE3)-lysate Polyclonal antibody (Cat# 10558-1-Ig) has 1 total citation. It appears in Endocrinology (2022), where it was used for Western blot in zebrafish. The cited study investigated vasoactive intestinal peptide's role in pituitary LH secretion independent of GnRH in female zebrafish, placing the research in the fields of endocrinology and reproductive biology.
| Species | Application | Title |
|---|---|---|
Endocrinology Vasoactive Intestinal Peptide Indirectly Elicits Pituitary LH Secretion Independent of GnRH in Female Zebrafish. |
