BLNK Polyclonal antibody

BLNK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10855-1-AP

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65



Tested Applications

Positive WB detected inRaji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10855-1-AP targets BLNK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLNK fusion protein Ag1290
Full Name B-cell linker
Calculated molecular weight 50 kDa
Observed molecular weight 68 kDa, 70 kDa
GenBank accession numberBC018906
Gene symbol BLNK
Gene ID (NCBI) 29760
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BLNK antibody 10855-1-APDownload protocol
IHC protocol for BLNK antibody 10855-1-APDownload protocol
IF protocol for BLNK antibody 10855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols