BLOC1S1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

BLOC1S1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 19687-1-AP

Synonyms

BLOC 1 subunit 1, BLOC1S1, BLOC1S1-Specific, BLOS1, GCN5 like protein 1, GCN5L1, MICoA, Protein RT14, RT14

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Validation Data Gallery

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  WB analysis of human brain using 19687-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19687-1-AP

  IP result of anti-BLOC1S1-Specific (IP:19687-1-AP, 3ug; Detection:19687-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human small intestine using 19687-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 19687-1-AP (BLOC1S1-Specific Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

19687-1-AP targets BLOC1S1-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: biogenesis of lysosomal organelles complex-1, subunit 1
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: NM_001487
• Gene Symbol: BLOC1S1
• Gene ID (NCBI): 2647
• RRID: AB_10666440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLOC1S1, also named as BLOS1, GCN5L1 and RT14, belongs to the BLOC1S1 family. BLOC1S1 may be involved in the biogenesis of specialized organelles of the endosomal-lysosomal system. The antibody is specific to NLOC1S1.

Publications

Species	Application	Title
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
human	WB	EMBO J; Snapin promotes HIV-1 transmission from dendritic cells by dampening TLR8 signaling.; Authors - Elham Khatamzas ; KD Validated
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
human	WB	Oxid Med Cell Longev; General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease.; Authors - Tingting Lv
	WB,IHC,IF,IP	FEBS J; A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.; Authors - Daria Sicari