BLVRB Polyclonal antibody

BLVRB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17727-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BVR-B, BVRB, BVR B, Biliverdin-IX beta-reductase, Biliverdin reductase B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 17727-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 17727-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of L02 using 17727-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat liver using 17727-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human liver using 17727-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 17727-1-AP (BLVRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17727-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 17727-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat liver using 17727-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17727-1-AP (BLVRB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17727-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17727-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17727-1-AP (BLVRB Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, human liver tissue, L02 cells, rat liver tissue
Positive IHC detected in	human liver tissue, rat kidney tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17727-1-AP targets BLVRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12124 Product name: Recombinant human BLVRB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC109371 Sequence: MAVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ Predict reactive species
Full Name	biliverdin reductase B (flavin reductase (NADPH))
Calculated Molecular Weight	206 aa, 22 kDa
Observed Molecular Weight	22-27 kDa
GenBank Accession Number	BC109371
Gene Symbol	BLVRB
Gene ID (NCBI)	645
RRID	AB_2065087
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30043
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for BLVRB antibody 17727-1-AP	Download protocol
WB protocol for BLVRB antibody 17727-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The BLVRB polyclonal antibody (Cat# 17727-1-AP) has 3 citations. These appear in Oxidative Medicine and Cell Longevity, EMBO Reports, and Cell Chemical Biology. The research fields span neuronal oxidative stress involving the Nrf2 pathway, hemoglobin clearance via liver sinusoidal endothelial cells, and cysteine-engagement profiling of oxidants.

Species	Application	Title
mouse	WB	Oxid Med Cell Longev Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway. Authors - Yinzhong Lu View Article
mouse,human,rat		EMBO Rep Liver sinusoidal endothelial cells constitute a major route for hemoglobin clearance Authors - Gabriela Zurawska View Article
		Cell Chem Biol A lysis-derived PMSF and orthovanadate oxidant defines a distinct cysteine-engagement profile. Authors - Bethany E. Schaffer View Article