BMP3 Polyclonal antibody

BMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22188-1-AP

Synonyms

BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin

---

Validation Data Gallery

• 

  WB analysis of Recombinant protein using 22188-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 22188-1-AP (BMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22188-1-AP (BMP3 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 22188-1-AP is BMP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMP3 fusion protein Ag16750
• Full Name: bone morphogenetic protein 3
• Calculated Molecular Weight: 472 aa, 53 kDa
• GenBank Accession Number: BC096269
• Gene Symbol: BMP3
• Gene ID (NCBI): 651
• RRID: AB_2879019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Bone morphogenetic protein-3 is a negative regulator of transforming growth factor beta and fibrosis; Authors - Emma R Dorris
human	WB	Biol Trace Elem Res; Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells.; Authors - Yan Wei