BMP4 Polyclonal antibody

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  WB analysis using 12492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12492-1-AP (BMP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12492-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12492-1-AP (BMP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 12492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 12492-1-AP (BMP4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 12492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BMP4 antibody (12492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

BMP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12492-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BMP 4, BMP-4, BMP2B, BMP-2B, Bone morphogenetic protein 2B

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

12492-1-AP targets BMP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMP4 fusion protein Ag3191
• Full Name: bone morphogenetic protein 4
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC020546
• Gene Symbol: BMP4
• Gene ID (NCBI): 652
• RRID: AB_2063531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12644
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMP4, also named as BMP2B and DVR4, belongs to the TGF-beta family. BMPs have been shown to promote astroglial differentiation both in vitro and in vivo. BMP4 have been shown to be components of serum and are thought to be responsible for this differentiation. BMP4 are expressed mainly in neurons, with minimal expression detected in astrocytes and oligodendrocytes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
mouse	IF	Theranostics; Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.; Authors - Wei Ge
human	IHC	Cancer Lett; SERPINE2 promotes esophageal squamous cell carcinoma metastasis by activating BMP4.; Authors - Jianglan Zhang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	WB,IHC	Am J Cancer Res; BMP4 augments the survival of hepatocellular carcinoma (HCC) cells under hypoxia and hypoglycemia conditions by promoting the glycolysis pathway.; Authors - Jiamin Zhong