Mouse BMP9 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86411-1-RR (same clone as 86411-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86411-1-RR (Gdf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86411-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02608-1

  The mean BMP9 concentration was determined to be 106.40 ng/mL in mouse liver tissue extract based on a 8.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02608-1

  Sandwich ELISA standard curve of MP02608-1, Mouse BMP9 Recombinant Matched Antibody Pair - PBS only. 86411-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2482. 86411-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

BMP9 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 86411-1-PBS

Clone No.250512B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

BMP-9, Bone morphogenetic protein 9, Gdf2, GDF-2, Growth/differentiation factor 2

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Product Information

86411-1-PBS targets BMP9 as part of a matched antibody pair:

MP02608-1: 86411-1-PBS capture and 86411-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2482

  Product name: recombinant mouse Gdf2 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 23-428 aa of NM_019506.4

  Sequence: KPLQNWEQASPGENAHSSLGLSGAGEEGVFDLQMFLENMKVDFLRSLNLSGIPSQDKTRAEPPQYMIDLYNRYTTDKSSTPASNIVRSFSVEDAISTAATEDFPFQKHILIFNISIPRHEQITRAELRLYVSCQNDVDSTHGLEGSMVVYDVLEDSETWDQATGTKTFLVSQDIRDEGWETLEVSSAVKRWVRADSTTNKNKLEVTVQSHRESCDTLDISVPPGSKNLPFFVVFSNDRSNGTKETRLELKEMIGHEQETMLVKTAKNAYQVAGESQEEEGLDGYTAVGPLLARRKRSTGASSHCQKTSLRVNFEDIGWDSWIIAPKEYDAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISILYKDDMGVPTLKYHYEGMSVAECGCR

• Full Name: growth differentiation factor 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: NM_019506.4
• Gene Symbol: BMP9
• Gene ID (NCBI): 12165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9WV56
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Bone morphogenetic protein-9 (BMP9, also termed growth-differentiation factor-2, GDF-2) is a secreted signaling molecule that belongs to the transforming growth factor-β (TGF-β) super-family. First identified in fetal mouse liver cDNA libraries, BMP9 is predominantly produced by hepatic stellate cells and circulates at relatively high concentrations in adult plasma. BMP9 is synthesized as a precursor protein (pre-proBMP9) composed of 429 amino acids (aa) that includes a 22 aa signal peptide, a 297 aa prodomain (33 kDa) and a 110 aa mature protein (12.5 kDa). It is then cleaved by serine endoproteases, leading to a short dimeric mature form of 25 kDa, and the prodomain which can remain noncovalently associated with the mature short form, giving a size of about 100 kDa (PMID:21710321).