BNC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BNC2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55220-1-AP

Synonyms

basonuclin 2, BNC2, BSN2

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Validation Data Gallery

• 

  WB analysis of HeLa using 55220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55220-1-AP (BNC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 55220-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 55220-1-AP (BNC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55220-1-AP (BNC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 2 publications below

Product Information

55220-1-AP targets BNC2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: basonuclin 2
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 122-140 kDa
• GenBank Accession Number: NM_017637
• Gene Symbol: BNC2
• Gene ID (NCBI): 54796
• RRID: AB_2881290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IP	Nat Commun; Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis; Authors - Marie Bobowski-Gerard ; KD Validated
human	IP	Cell Death Dis; O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in myofibroblasts; Authors - Ninon Very