BNIP1 Polyclonal antibody

BNIP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15964-1-AP

Synonyms

BNIP1, NIP1, SEC20, SEC20L, TRG 8



Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15964-1-AP targets BNIP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNIP1 fusion protein Ag8737
Full Name BCL2/adenovirus E1B 19kDa interacting protein 1
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC010959
Gene Symbol BNIP1
Gene ID (NCBI) 662
RRIDAB_2066636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.

Protocols

Product Specific Protocols
WB protocol for BNIP1 antibody 15964-1-APDownload protocol
IHC protocol for BNIP1 antibody 15964-1-APDownload protocol
IP protocol for BNIP1 antibody 15964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Signal

A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.

Authors - Wang Peng P
humanWB,IF

Hum Mutat

A homozygous hypomorphic BNIP1 variant causes an increase in autophagosomes and reduced autophagic flux and results in a spondylo-epiphyseal dysplasia.

Authors - Tess Holling
humanWB,IF

PLoS One

RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.

Authors - Tang Fei F
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss