|Positive WB detected in||HeLa cells, mouse skeletal muscle tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15964-1-AP targets BNIP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BNIP1 fusion protein Ag8737|
|Full Name||BCL2/adenovirus E1B 19kDa interacting protein 1|
|Calculated molecular weight||228 aa, 26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC010959|
|Gene ID (NCBI)||662|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.
RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.
A homozygous hypomorphic BNIP1 variant causes an increase in autophagosomes and reduced autophagic flux and results in a spondylo-epiphyseal dysplasia.
A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.