BNIPL Polyclonal antibody

BNIPL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13262-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein, BNIP S, BNIPL 1, BNIPL 2, BNIPL1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 13262-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 13262-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 13262-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 13262-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse skin tissue, rat skin tissue
Positive IHC detected in	mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13262-1-AP targets BNIPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3921 Product name: Recombinant human BNIPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC027868 Sequence: MGTIQEAGKKTDVGVREIAEAPELGAALRHGELELKEEWQDEEFPRLLPEEAGTSEDPEDPKGDSQAAAGTPSTLALCGQRPMRKRLSAPELRLSLTKGPGNDGASPTQSAPSSPDGSSDLEIDELETPSDSEQLDSGHEFEWEDELPRAEGLGTSETAERLGRGCMWDVTGEDGHHWRVFRMGPREQRVDMTVIEPYKKVLSHGGYHGDGLNAVILFASCYLPRSSIPNYTYVMEHLFRYMVGTLELLVAENYLLVHLSGGTSRAQVPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLALLRPFISSKFTRKIRFLDSLGELAQLISLDQVHIPEAVRQLDRDLHGSGGT Predict reactive species
Full Name	BCL2/adenovirus E1B 19kD interacting protein like
Calculated Molecular Weight	357 aa, 40 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	BC027868
Gene Symbol	BNIPL
Gene ID (NCBI)	149428
RRID	AB_3085410
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z465
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.

Protocols

Product Specific Protocols
IHC protocol for BNIPL antibody 13262-1-AP	Download protocol
WB protocol for BNIPL antibody 13262-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The BNIPL polyclonal antibody (Cat# 13262-1-AP) has 1 total citation. It appears in the International Journal of Biological Sciences (IF: 11.7), published in January 2026. The cited study investigated how FOXO3 upregulates and activates GSDME to trigger pyroptosis in myeloma cells, placing this citation within the fields of programmed cell death (pyroptosis) and hematologic oncology. The antibody was used for Western blot analysis in human samples with RNAi validation.