BNIPL Polyclonal antibody

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  WB analysis using 13262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 13262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 13262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BNIPL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13262-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein, BNIP S, BNIPL 1, BNIPL 2, BNIPL1

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13262-1-AP targets BNIPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3921

  Product name: Recombinant human BNIPL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-357 aa of BC027868

  Sequence: MGTIQEAGKKTDVGVREIAEAPELGAALRHGELELKEEWQDEEFPRLLPEEAGTSEDPEDPKGDSQAAAGTPSTLALCGQRPMRKRLSAPELRLSLTKGPGNDGASPTQSAPSSPDGSSDLEIDELETPSDSEQLDSGHEFEWEDELPRAEGLGTSETAERLGRGCMWDVTGEDGHHWRVFRMGPREQRVDMTVIEPYKKVLSHGGYHGDGLNAVILFASCYLPRSSIPNYTYVMEHLFRYMVGTLELLVAENYLLVHLSGGTSRAQVPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLALLRPFISSKFTRKIRFLDSLGELAQLISLDQVHIPEAVRQLDRDLHGSGGT

• Full Name: BCL2/adenovirus E1B 19kD interacting protein like
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC027868
• Gene Symbol: BNIPL
• Gene ID (NCBI): 149428
• RRID: AB_3085410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z465
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.