BNIPL Polyclonal antibody

BNIPL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13262-1-AP

Synonyms

BNIP S, BNIPL, BNIPL 1, BNIPL 2, BNIPL1, BNIPL2, PP753

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Validation Data Gallery

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  WB analysis using 13262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13262-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13262-1-AP (BNIPL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 13262-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13262-1-AP (BNIPL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 13262-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13262-1-AP (BNIPL antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13262-1-AP targets BNIPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BNIPL fusion protein Ag3921
• Full Name: BCL2/adenovirus E1B 19kD interacting protein like
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC027868
• Gene Symbol: BNIPL
• Gene ID (NCBI): 149428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.