BORIS Polyclonal antibody

BORIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11074-2-AP

Synonyms

CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27

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Validation Data Gallery

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  WB analysis of PC-3 using 11074-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 11074-2-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11074-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 11074-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11074-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11074-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BORIS antibody (11074-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11074-2-AP targets BORIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BORIS fusion protein Ag1547
• Full Name: CCCTC-binding factor (zinc finger protein)-like
• Calculated Molecular Weight: 82 kDa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC130486
• Gene Symbol: BORIS/CTCFL
• Gene ID (NCBI): 140690
• RRID: AB_614983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. Present polyclonal anti-CFCFL antibody(11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detect a 75-kDa band and a 55-kDa band.