BPGM Polyclonal antibody

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  WB analysis using 17173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 17173-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17173-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17173-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17173-1-AP

  IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

BPGM Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17173-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

2,3 bisphosphoglycerate mutase, 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate synthase, 2,3-diphosphoglycerate mutase, Bisphosphoglycerate mutase

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Tested Applications

• Positive WB detected in: HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17173-1-AP targets BPGM in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10731

  Product name: Recombinant human BPGM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-259 aa of BC017050

  Sequence: MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK

• Full Name: 2,3-bisphosphoglycerate mutase
• Calculated Molecular Weight: 259 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC017050
• Gene Symbol: BPGM
• Gene ID (NCBI): 669
• RRID: AB_2274991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07738
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BPGM is a cytosolic erythrocyte enzyme that controls intracellular levels of 2,3-bisphosphoglycerate, a key allosteric regulator of hemoglobin. By modulating hemoglobin oxygen affinity, BPGM ensures efficient oxygen delivery to tissues and supports physiological adaptation to hypoxia. Genetic disruption of BPGM leads to erythrocytosis and impaired oxygen release, underscoring its central role in red blood cell metabolic specialization. BPGM in HCC cells also induced both histone lactylation and M2 polarization of macrophages by lactate secretion. (PMID: 41514495)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP	Cell Metab; Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.; Authors - Ping Xu
		Adv Sci (Weinh); Hepatocyte BPGM Induces RET Lactylation and Macrophage Reprogramming to Promote Tumorigenesis in Hepatocellular Carcinoma; Authors - Jiajia Zhang
rat	WB,IF	Brain Res Bull; Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2; Authors - Guoji E
rat	WB	Front Physiol; Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia; Authors - Song Yu
human	WB	Aging Cell; Longevity Humans Have Youthful Erythrocyte Function and Metabolic Signatures; Authors - Fang Yu