BPIL1 Polyclonal antibody

BPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13461-2-AP

Synonyms

Long palate, lung and nasal epithelium carcinoma-associated protein 2, C20orf184, BPI-like 1, BPIFB2, BPI fold-containing family B member 2

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Validation Data Gallery

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  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13461-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13461-2-AP (BPIL1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13461-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.

• 

  IF Staining of A549 using 13461-2-AP

  Immunofluorescent analysis of A549 cells using 13461-2-AP (BPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

13461-2-AP targets BPIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BPIL1 fusion protein Ag4051
• Full Name: bactericidal/permeability-increasing protein-like 1
• Calculated Molecular Weight: 458 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC034415
• Gene Symbol: BPIL1
• Gene ID (NCBI): 80341
• RRID: AB_2067089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein which may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).

Publications

Species	Application	Title
human	WB,IHC	Allergy; Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.; Authors - Seshadri S S
human	WB	J Proteome Res; Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.; Authors - Foster Matthew W MW
human	IHC	J Immunol Res; Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.; Authors - Yanran Huang