BPNT1 Polyclonal antibody

BPNT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16461-1-AP

Synonyms

Bisphosphate 3 nucleotidase 1, BPNT1, PAP inositol 1,4 phosphatase, PIP

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Validation Data Gallery

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  WB analysis of HEK-293 using 16461-1-AP

  HEK-293 and Hela cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16461-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16461-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16461-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16461-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16461-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16461-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16461-1-AP (BPNT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human kidney tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16461-1-AP targets BPNT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BPNT1 fusion protein Ag9523
• Full Name: 3'(2'), 5'-bisphosphate nucleotidase 1
• Calculated Molecular Weight: 325 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC017801
• Gene Symbol: BPNT1
• Gene ID (NCBI): 10380
• RRID: AB_2067193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	PLoS One; Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea; Authors - Jing Liu