BRAP Polyclonal antibody

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  WB analysis of MCF-7 using 26004-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26004-1-AP (BRAP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 26004-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26004-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 40x lens).

BRAP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26004-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26004-1-AP targets BRAP in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23279

  Product name: Recombinant human BRAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-200 aa of BC136698

  Sequence: AQRSKDHSKECINAAPDSPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCILTVPAAMTSHDLMKFVAPFNEVIEQMKIIRDSTPN

• Full Name: BRCA1 associated protein
• Calculated Molecular Weight: 592 aa, 67 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC136698
• Gene Symbol: BRAP
• Gene ID (NCBI): 8315
• RRID: AB_2880330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z569
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.