BRD1/BRL Polyclonal antibody

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  WB analysis using 32452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32452-1-AP (BRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

BRD1/BRL Polyclonal Antibody for WB, ELISA

Cat No. 32452-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells, SK-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32452-1-AP targets BRD1/BRL in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRD1/BRL fusion protein Ag37530
• Full Name: bromodomain containing 1
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC047508
• Gene Symbol: BRD1
• Gene ID (NCBI): 23774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95696
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa.