BRD3 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA sample test of MP02845-2

  The mean BRD3 concentration was determined to be 16.21 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 8.56 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02845-2

  Sandwich ELISA standard curve of MP02845-2, Human BRD3 Recombinant Matched Antibody Pair - PBS only. 87030-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2433. 87030-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Cytometric bead array sample test of MP02845-1

  The mean BRD3 concentration was determined to be 36.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 32.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02845-1

  Cytometric bead array standard curve of MP02845-1, BRD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87030-2-PBS. Detection antibody: 87030-1-PBS. Standard: Ag2433. Range: 0.312-40 ng/mL.

BRD3 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 87030-2-PBS

Clone No.252143B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

---

Product Information

87030-2-PBS targets BRD3 as part of a matched antibody pair:

MP02845-1: 87030-2-PBS capture and 87030-1-PBS detection (validated in Cytometric bead array)

MP02845-2: 87030-2-PBS capture and 87030-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2433

  Product name: Recombinant human BRD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC032124

  Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM

• Full Name: bromodomain containing 3
• Calculated Molecular Weight: 556 aa, 61 kDa
• GenBank Accession Number: BC032124
• Gene Symbol: BRD3
• Gene ID (NCBI): 8019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15059
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.