Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, Jurkat cells, MCF-7 cells, Ramos cells, MDA-MB-231 cells, T-47D cells, SGC-7901 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28486-1-AP targets BRD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | bromodomain containing 4 |
| Calculated Molecular Weight | 1362 aa, 152 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC035266 |
| Gene Symbol | BRD4 |
| Gene ID (NCBI) | 23476 |
| RRID | AB_2918170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60885 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. This antibody detects the small and the long isoform of BRD4, the molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BRD4 antibody 28486-1-AP | Download protocol |
| IHC protocol for BRD4 antibody 28486-1-AP | Download protocol |
| IP protocol for BRD4 antibody 28486-1-AP | Download protocol |
| WB protocol for BRD4 antibody 28486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BRD4 Polyclonal antibody (catalog number 28486-1-AP) has 23 citations. These appear in journals including Theranostics, Cell Reports, Oncogene, Journal of Controlled Release, Cell Communications & Signaling, Phytomedicine, Journal of Translational Medicine, Archives of Pharmacal Research, Journal of Medicinal Chemistry, Frontiers in Immunology, Cell & Molecular Life Sciences, Biochemical Pharmacology, Communications Biology, Frontiers in Pharmacology, Scientific Reports, RSC Chemical Biology, Frontiers in Medicine, Tissue & Cell, PLoS One, bioRxiv, and European Journal of Medicinal Chemistry. Research fields span cancer (breast, gastric, glioma, AML), fibrosis, immunology, epigenetics, and protein degradation.
| Species | Application | Title |
|---|---|---|
Theranostics A novel ROR1-targeting antibody-PROTAC conjugate promotes BRD4 degradation for solid tumor treatment | ||
Cell Rep Med An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer | ||
J Control Release The HSP90-dependent bioorthogonal PROTAC prodrug system enables tumor-selective and enhanced protein degradation. | ||
Cell Commun Signal Specific sDMA modifications on the RGG/RG motif of METTL14 regulate its function in AML | ||
Phytomedicine Huangqi-Danshen Decoction Alleviates Cardiac Fibrosis Following Myocardial Infarction by Inhibiting USP22 to Enhance the Ubiquitination of BRD4. | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation |
Reviews
What customers say
The single review for this product is a 5-star rating. The user performed Western Blot on HeLa cells at a 1:1000 dilution and reported that the antibody successfully detected three BRD4 bands, indicating strong specificity and performance for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (06-13-2024) | detects 3 BRD4 bands in HeLa cells
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