BRD4 Monoclonal antibody

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• BRD4 Monoclonal antibody (67374-2-Ig)

BRD4 Monoclonal Antibody for WB, ELISA

Cat No. 67374-2-Ig Copied!

Clone No. 2F7E2

Publications (16)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (3)

Applications

WB, IHC, IF, ChIP, ELISA

Synonyms

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

• 

  WB analysis using 67374-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 67374-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67374-2-Ig targets BRD4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27545

  Product name: Recombinant human BRD4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 311-633 aa of BC035266

  Sequence: PEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRV

• Full Name: bromodomain containing 4
• Calculated Molecular Weight: 1362 aa, 152 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC035266
• Gene Symbol: BRD4
• Gene ID (NCBI): 23476
• RRID: AB_2918488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60885
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The BRD4 Monoclonal antibody (Cat# 67374-2-Ig) has 16 citations published in journals including Cell, Nature Methods, Nature Communications, Cell Death & Disease, Cell Reports, BMC Biology, European Journal of Medicinal Chemistry, Journal of Proteome Research, and others. Research fields span epigenetics, transcriptional regulation, cancer biology (breast, prostate, colorectal, lung, gastric, bladder, glioblastoma, thyroid, and head & neck cancers), ferroptosis, drug discovery, immune evasion, and inflammatory responses. Applications include WB, ChIP, IF, and IHC.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Cell; De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses; Authors - Xiao-Qi Liu
		Nat Methods; High-throughput profiling of chemical-induced gene expression across 93,644 perturbations; Authors - Lei Xiang
human	WB	Nat Commun; Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes; Authors - Caiping Tian
mouse	WB	Nat Commun; MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition; Authors - Fan Tong
rat	ChIP	Cell Death Dis; (+)-JQ-1 alleviates cardiac injury in myocardial infarction by inhibiting ferroptosis through the NAMPT/SIRT1 pathway.; Authors - Mengxue Yang
human		J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan