BRD4 Recombinant monoclonal antibody

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• BRD4 Recombinant monoclonal antibody (88289-1-RR)

BRD4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88289-1-RR

Clone No.260746G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP

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  WB analysis using 88289-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88289-1-RR (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88289-1-RR targets BRD4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: bromodomain containing 4
• Calculated Molecular Weight: 1362 aa, 152 kDa
• Observed Molecular Weight: 200-240 kDa
• GenBank Accession Number: BC035266
• Gene Symbol: BRD4
• Gene ID (NCBI): 23476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60885
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members.