BRD7 Recombinant antibody

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  WB analysis using 86176-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 86176-2-RR

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 86176-2-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 86176-2-RR (BRD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

BRD7 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86176-2-RR

Clone No.250776B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse lung tissue, PC-3 cells, SH-SY5Y cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86176-2-RR targets BRD7 in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BRD7 fusion protein Ag1461
• Full Name: bromodomain containing 7
• Calculated Molecular Weight: 651 aa, 74 kDa
• Observed Molecular Weight: 74-78 kDa
• GenBank Accession Number: BC050728
• Gene Symbol: BRD7
• Gene ID (NCBI): 29117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.