BRD9 Polyclonal antibody

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  WB analysis of HeLa using 24785-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24785-1-AP (BRD9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24785-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24785-1-AP (BRD9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 24785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24785-1-AP (BRD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24785-1-AP (BRD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BRD9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24785-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

BRD 9, Bromodomain-containing protein 9, LAVS3040, Rhabdomyosarcoma antigen MU-RMS-40.8, UNQ3040/PRO9856

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

24785-1-AP targets BRD9 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20427

  Product name: Recombinant human BRD9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 133-481 aa of BC041590

  Sequence: TAVEEPVPEVVPVQVETAKKSKKPSREVISCMFEPEGNACSLTDSTAEEHVLALVEHAADEARDRINRFLPGGKMGYLKRNGDGSLLYSVVNTAEPDADEEETHPVDLSSLSSKLLPGFTTLGFKDERRNKVTFLSSATTALSMQNNSVFGDLKSDEMELLYSAYGDETGVQCALSLQEFVKDAGSYSKKVVDDLLDQITGGDHSRTLFQLKQRRNVPMKPPDEAKVGDTLGDSSSSVLEFMSMKSYPDVSVDISMLSSLGKVKKELDPDDSHLNLDETTKLLQDLHEAQAERGGSRPSSNLSSLSNASERDQHHLGSPSRLSVGEQPDVTHDPYEFLQSPEPAASAKT

• Full Name: bromodomain containing 9
• Calculated Molecular Weight: 597 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC041590
• Gene Symbol: BRD9
• Gene ID (NCBI): 65980
• RRID: AB_2879722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H8M2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription. BRD9 has six isoforms with MW 67 kDa, 23 kDa, 53 kDa, 26 kDa, 56 kDa and 61 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
human	WB	Nat Commun; SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors; Authors - Ning Qing Liu
human	IP,IF	PLoS Pathog; Merkel cell polyomavirus small T antigen is a viral transcription activator that is essential for viral genome maintenance; Authors - Kyle Rapchak
human	WB	J Hematol Oncol; Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation; Authors - Pengyun Li
rat	WB	Neuroscience; Scalp acupuncture alleviates remote hippocampal damage in MCAO rats by inhibiting neuroinflammation: A TMT-based proteomics analysis; Authors - Huacong Liu