BRN3A Polyclonal antibody

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• BRN3A Polyclonal antibody (32257-1-AP)

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  WB analysis using 32257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32257-1-AP (BRN3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 32257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 32257-1-AP (BRN3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat eye using 32257-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using BRN3A antibody (32257-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BRN3A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 32257-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Brain-3A, Brain-specific homeobox/POU domain protein 3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32257-1-AP targets BRN3A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35800

  Product name: Recombinant human BRN3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 87-265 aa of NM_006237

  Sequence: SVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDP

• Full Name: POU class 4 homeobox 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_006237
• Gene Symbol: POU4F1
• Gene ID (NCBI): 5457
• RRID: AB_3742554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q01851
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRN3A (Brain-specific Homeobox/POU domain protein 3A, also known as POU4F1) is a member of the class IV POU-domain transcription factor family. It plays a core regulatory role primarily in the development and survival of the nervous system and sensory neurons. By recognizing specific DNA sequences, it activates downstream target genes and is essential for the generation, differentiation, and maintenance of sensory ganglion neurons, including those of the trigeminal, vestibular, and auditory systems. Additionally, it is also involved in regulating cell proliferation and apoptosis in tumors such as breast cancer. It has 2 isoforms with the molecular mass of 33 kDa and 43 kDa.