MPC2 Polyclonal antibody

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• MPC2 Polyclonal antibody (20049-1-AP)

MPC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20049-1-AP Copied!

Publications (22)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Brain protein 44, BRP44, Mitochondrial pyruvate carrier 2

• 

  WB analysis of mouse brain using 20049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293T using 20049-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 20049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20049-1-AP (MPC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 20049-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20049-1-AP (MPC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, LNCaP cells, PC-3 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20049-1-AP targets MPC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: brain protein 44
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_015415
• Gene Symbol: MPC2
• Gene ID (NCBI): 25874
• RRID: AB_10665371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95563
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRP44 belongs to the UPF0041 (0-44) family. The antibody is specific to BRP44.

Publications

What published studies show

The MPC2 polyclonal antibody (Cat# 20049-1-AP) has 22 citations across 21 journals, including Mol Cancer, Curr Biol, J Biol Chem, EMBO Rep, Metabolism, and Cell Death Dis. Research fields span cancer metabolism, mitochondrial biology, neurodegeneration (ALS, Rett syndrome), diabetes, bone biology, reproductive biology, and stroke. Cited applications include WB, IF, and IHC in human, mouse, and rat models.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cancer; 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants.; Authors - Eumorphia G Konstantakou
mouse	WB	Metabolism; Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models; Authors - Stephanie Zur Nedden
mouse	WB	Cell Death Dis; RB1 controls differentiation through positive regulation of phosphoglycerate mutases; Authors - Susumu Kohno
mouse	WB	Aging Dis; Inhibiting Glutaminase Exerts Opposite Effects on Ovariectomy-Induced and Age-Related Reductions in Murine Bone Mass; Authors - Qian Guo
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
rat	WB	Clin Sci (Lond); LONP1 facilitates pulmonary artery smooth muscle cell glycolytic reprogramming by degrading MPC1 in pulmonary hypertension; Authors - Mingkang Li