MPC2 Polyclonal antibody

MPC2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 20049-1-AP

Synonyms

brain protein 44, BRP44, Mitochondrial pyruvate carrier 2, MPC2

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Validation Data Gallery

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  WB analysis of mouse brain using 20049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 20049-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20049-1-AP (MPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20049-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20049-1-AP (MPC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, LNCaP cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

20049-1-AP targets MPC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: brain protein 44
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_015415
• Gene Symbol: MPC2
• Gene ID (NCBI): 25874
• RRID: AB_10665371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRP44 belongs to the UPF0041 (0-44) family. The antibody is specific to BRP44.

Publications

Species	Application	Title
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
human	WB	Mol Cancer; 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants.; Authors - Eumorphia G Konstantakou
mouse	WB	J Biol Chem; Mitochondrial pyruvate carrier blockade results in decreased osteoclastogenesis and bone resorption via regulating mitochondrial energy production.; Authors - Qian Guo
human	WB	Hum Mol Genet; Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants; Authors - Stephanie A Zlatic
human	IF	Life Sci; Mitochondrial pyruvate carrier 2 mediates mitochondrial dysfunction and apoptosis in high glucose-treated podocytes.; Authors - Jun Feng
rat	WB,IF	Brain Res Bull; Downregulation of mitochondrial pyruvate carrier 2 aggravates neuronal injury in the cortex following cerebral ischemia in rat.; Authors - Yue Wang