BRUNOL5 Polyclonal antibody

BRUNOL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13557-1-AP

Synonyms

Bruno like protein 5, BRUNOL 5, BRUNOL5, CELF 5, CELF5, RNA binding protein BRUNOL 5

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Validation Data Gallery

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  WB analysis using 13557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13557-1-AP (BRUNOL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13557-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 13557-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13557-1-AP targets BRUNOL5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRUNOL5 fusion protein Ag4416
• Full Name: bruno-like 5, RNA binding protein (Drosophila)
• Calculated Molecular Weight: 485 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC028101
• Gene Symbol: BRUNOL5
• Gene ID (NCBI): 60680
• RRID: AB_1211624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRUNOL5, also named CELF5, is a 485 amino acid protein, which belongs to the CELF/BRUNOL family. BRUNOL5 is expressed in the brain and localizes in the nucleus. BRUNOL5 is an RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. BRUNOL5 specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. BRUNOL5 exists in two isoforms with MV 52 and 45 kDa.