Tested Applications
| Positive WB detected in | bsa, Bovine serum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66201-1-Ig targets BSA in WB, IF, ELISA applications and shows reactivity with bovine samples.
| Tested Reactivity | bovine |
| Cited Reactivity | human, mouse, bovine, pcn033 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Natural Protein Predict reactive species |
| Full Name | bovine serum albumin |
| Observed Molecular Weight | 67 kDa |
| Gene Symbol | BSA |
| Gene ID (NCBI) | 280717 |
| RRID | AB_2881592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BSA, also named as ALB and Bovine Serum albumin, is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BSA antibody 66201-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
BSA Monoclonal antibody (2D11G3) (Cat# 66201-1-Ig) has 24 citations published in journals including Cell Metabolism, Nature Communications, Science Advances, Redox Biology, Advanced Science, Journal of Nanobiotechnology, Journal of Neuroinflammation, ACS Sensors, Acta Biomaterialia, Developmental Cell, International Journal of Nanomedicine, NPJ Parkinson's Disease, Frontiers in Microbiology, Journal of Virology, Molecular Immunology, Experimental Hematology, and bioRxiv. Research fields span cancer, extracellular vesicles, neuroinflammation, infectious diseases, hematology, aging, and drug resistance.
| Species | Application | Title |
|---|---|---|
Cell Metab RIPK1 senses S-adenosylmethionine scarcity to drive cell death and inflammation | ||
Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. | ||
Nat Commun Internalized components of membrane attack complexes disrupt proteostasis and acquire alarmin-like properties. | ||
Redox Biol Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression | ||
J Nanobiotechnology Protective role of small extracellular vesicles derived from HUVECs treated with AGEs in diabetic vascular calcification | ||
Adv Sci (Weinh) CD147-K148me2-Driven Tumor Cell-Macrophage Crosstalk Provokes NSCLC Immunosuppression via the CCL5/CCR5 Axis |
Reviews
What customers say
Both reviewers gave 5-star ratings for Western Blot applications. One user at 1:5,000 dilution on primary mouse neurons reported the antibody perfectly recognized an internalized BSA-conjugated probe. Another at 1:10,000 on BV-2 cell supernatant described clean results with correct band detection and overall excellent performance. In summary, customers find this antibody reliable, specific, and easy to use for WB, with no issues reported across different sample types and dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ning (Verified Customer) (02-27-2023) | Can perfectly recognize the internalized BSA-conjugated probe.
|
Jake (Verified Customer) (08-25-2021) | Overall the antibody worked great. It was clean, detected the correct band and worked with my samples. Very Happy with product
|
