BSA Monoclonal antibody

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  WB analysis using 66201-1-Ig

  20ng, 10ng and 5ng BSA were subjected to SDS PAGE followed by western blot with 66201-1-Ig (BSA Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of bovine serum using 66201-1-Ig

  Bovine serum were subjected to SDS PAGE followed by western blot with 66201-1-Ig (BSA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

BSA Monoclonal Antibody for WB, ELISA

Cat No. 66201-1-Ig

Clone No.2D11G3

Publications(18)

Reviews (2)

Host / Isotype

Mouse / IgG1

Reactivity

bovine and More (3)

Applications

WB, IF, ELISA

Synonyms

ALB, Albumin, Bos d 6, Bos Serum albumin, Bovine Serum albumin

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Tested Applications

• Positive WB detected in: bsa, Bovine serum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IF: See 1 publications below

Product Information

66201-1-Ig targets BSA in WB, IF, ELISA applications and shows reactivity with bovine samples.

• Tested Reactivity: bovine
• Cited Reactivity: human, mouse, pcn033
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Natural Protein

• Full Name: bovine serum albumin
• Observed Molecular Weight: 67 kDa
• Gene Symbol: BSA
• Gene ID (NCBI): 280717
• RRID: AB_2881592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BSA, also named as ALB and Bovine Serum albumin, is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
	WB	Redox Biol; Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression; Authors - Cristina Mas-Bargues
	WB	Dev Cell; Transnitrosylation Mediated by the Non-canonical Catalase ROG1 Regulates Nitric Oxide Signaling in Plants.; Authors - Lichao Chen
human	WB	Int J Nanomedicine; Exosomal Vaccine Loading T Cell Epitope Peptides of SARS-CoV-2 Induces Robust CD8+ T Cell Response in HLA-A Transgenic Mice; Authors - An-Ran Shen
human	WB	J Nanobiotechnology; Protective role of small extracellular vesicles derived from HUVECs treated with AGEs in diabetic vascular calcification; Authors - Bei Guo
mouse	WB	J Virol; Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment; Authors - Jake T Mills

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ning (Verified Customer) (02-27-2023)

Can perfectly recognize the internalized BSA-conjugated probe.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: primary mouse neurons

FH

Jake (Verified Customer) (08-25-2021)

Overall the antibody worked great. It was clean, detected the correct band and worked with my samples. Very Happy with product

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: BV-2 Cells (supernatant)