CD157 Polyclonal antibody

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  WB analysis of A549 using 16337-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16337-1-AP (CD157 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 16337-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16337-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD157 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16337-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

ADP ribosyl cyclase 2, Bone marrow stromal antigen 1, BST 1, BST1, cADPr hydrolase 2, CD157, Cyclic ADP ribose hydrolase 2

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16337-1-AP targets CD157 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD157 fusion protein Ag9491
• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 318 aa, 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC012095
• Gene Symbol: CD157
• Gene ID (NCBI): 683
• RRID: AB_2878244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q10588
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. BST-1 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to the glycosylation (PMID: 8941363, 10491089).

Publications

Species	Application	Title
rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang