CD157 Polyclonal antibody

CD157 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16337-1-AP

Synonyms

ADP ribosyl cyclase 2, Bone marrow stromal antigen 1, BST 1, BST1, cADPr hydrolase 2, CD157, Cyclic ADP ribose hydrolase 2

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Validation Data Gallery

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  WB analysis of A549 using 16337-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16337-1-AP (CD157 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 16337-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16337-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16337-1-AP (CD157 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16337-1-AP targets CD157 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD157 fusion protein Ag9491
• Full Name: bone marrow stromal cell antigen 1
• Calculated Molecular Weight: 318 aa, 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC012095
• Gene Symbol: CD157
• Gene ID (NCBI): 683
• RRID: AB_2878244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. BST-1 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to the glycosylation (PMID: 8941363, 10491089).

Publications

Species	Application	Title
rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang