CD157 Polyclonal antibody

CD157 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16337-1-AP

Synonyms

ADP ribosyl cyclase 2, Bone marrow stromal antigen 1, BST 1, BST1, cADPr hydrolase 2, CD157, Cyclic ADP ribose hydrolase 2



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16337-1-AP targets CD157 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD157 fusion protein Ag9491
Full Name bone marrow stromal cell antigen 1
Calculated molecular weight 318 aa, 36 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC012095
Gene symbol BST1
Gene ID (NCBI) 683
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST-1(bone marrow stromal cell antigen 1), also named as CD157 or ADP-ribosyl cyclase 2, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. BST-1 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to the glycosylation (PMID: 8941363, 10491089).

Protocols

Product Specific Protocols
WB protocol for CD157 antibody 16337-1-APDownload protocol
IHC protocol for CD157 antibody 16337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols