BST2 Monoclonal antibody

BST2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3C4B5

Cat no : 66919-1-Ig

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, Human peripheral blood leukocyte cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66919-1-Ig targets BST2 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 18 kDa, 30-36 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 66919-1-IgDownload protocol
IHC protocol for BST2 antibody 66919-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols