BTBD1 Polyclonal antibody

BTBD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15859-1-AP

Synonyms

BTB (POZ) domain containing 1, BTBD1, C15orf1, NS5ATP8



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15859-1-AP targets BTBD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTBD1 fusion protein Ag8339
Full Name BTB (POZ) domain containing 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC028097
Gene Symbol BTBD1
Gene ID (NCBI) 53339
RRIDAB_2067248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BTBD1 antibody 15859-1-APDownload protocol
IHC protocol for BTBD1 antibody 15859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors

Authors - Alice Meroni