BTBD1 Polyclonal antibody

BTBD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15859-1-AP

Synonyms

BTB (POZ) domain containing 1, BTBD1, C15orf1, NS5ATP8

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Validation Data Gallery

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  WB analysis of HeLa using 15859-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15859-1-AP (BTBD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15859-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15859-1-AP (BTBD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15859-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15859-1-AP (BTBD1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15859-1-AP targets BTBD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTBD1 fusion protein Ag8339
• Full Name: BTB (POZ) domain containing 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC028097
• Gene Symbol: BTBD1
• Gene ID (NCBI): 53339
• RRID: AB_2067248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors; Authors - Alice Meroni