BTBD6 Polyclonal antibody

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  WB analysis of HepG2 using 13776-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13776-1-AP (BTBD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 13776-1-AP

  IP result of anti-BTBD6 (IP:13776-1-AP, 4ug; Detection:13776-1-AP 1:300) with Y79 cells lysate 800ug.

BTBD6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13776-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13776-1-AP targets BTBD6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4766

  Product name: Recombinant human BTBD6 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-410 aa of BC042525

  Sequence: MFNNELMADVHFVVGPPGATRTVPAHKYVLAVGSSVFYAMFYGDLAEVKSEIHIPDVEPAAFLILLKYMYSDEIDLEADTVLATLYAAKKYIVPALAKACVNFLETSLEAKNACVLLSQSRLFEEPELTQRCWEVIDAQAEMALRSEGFCEIDRQTLEIIVTREALNTKEAVVFEAVLNWAEAECKRQGLPITPRNKGHVLGRALYLVRIPTMTLEEFANGAAQSDILTLEETHSIFLWYTATNKPRLDFPLTKRKGLAPQRCHRFQSSAYRSNQWRYRGRCDSIQFAVDRRVFIAGLGLYGSSSGKAEYSVKIELKRLGVVLAQNLTKFMSDGSSNTFPVWFEHPVQVEQDTFYTASAVLDGSELSYFGQEGMTEVQCGKVAFQFQCSSDSTNGTGVQGGQIPELIFYA

• Full Name: BTB (POZ) domain containing 6
• Calculated Molecular Weight: 410 aa, 46 kDa
• Observed Molecular Weight: 53-58 kDa
• GenBank Accession Number: BC042525
• Gene Symbol: BTBD6
• Gene ID (NCBI): 90135
• RRID: AB_2877975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96KE9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.