BTBD8 Polyclonal antibody

BTBD8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23897-1-AP

Synonyms

BTB (POZ) domain containing 8, BTBD8

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Validation Data Gallery

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  WB analysis of HEK-293 using 23897-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23897-1-AP (BTBD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23897-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23897-1-AP (BTBD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23897-1-AP is BTBD8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTBD8 fusion protein Ag20960
• Full Name: BTB (POZ) domain containing 8
• Calculated Molecular Weight: 278 aa, 43 kDa
• Observed Molecular Weight: 43 kDa, 34 kDa
• GenBank Accession Number: BC013922
• Gene Symbol: BTBD8
• Gene ID (NCBI): 284697
• RRID: AB_2879353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTBD8, also named as BTB/POZ domain-containing protein 8, is a 378 amino acid protein, which is highly expressed in fetal brain and localizes in the nucleus. BTBD8 exists two isoforms and the molecular weight of isoforms are 43 kDa and 35 kDa.