BTC Monoclonal antibody

BTC Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2H5E8

Cat no : 66683-1-Ig

Synonyms

betacellulin, BTC, Probetacellulin



Tested Applications

Positive WB detected inMCF-7 cells, NCCIT cells, A549 cells, MDA-MB-231 cells, T-47D cells, PC-3 cells, BxPC-3 cells, DU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66683-1-Ig targets BTC in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BTC fusion protein Ag26724
Full Name betacellulin
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011618
Gene symbol BTC
Gene ID (NCBI) 685
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.

Protocols

Product Specific Protocols
WB protocol for BTC antibody 66683-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols