BTD Recombinant antibody

BTD Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84613-2-RR
Clone No.242057C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Biotinase, biotinidase, EC:3.5.1.12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84613-2-RR targets BTD in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BTD fusion protein Ag9449 Predict reactive species
Full Name biotinidase
Calculated Molecular Weight 543 aa, 61 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC012099
Gene Symbol BTD
Gene ID (NCBI) 686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43251
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in the regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

Protocols

Product Specific Protocols
WB protocol for BTD antibody 84613-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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