BTK Polyclonal antibody

BTK Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21581-1-AP

Synonyms

AGMX1, AT, ATK, B cell progenitor kinase, BPK, Bruton tyrosine kinase, BTK, IMD1, PSCTK1, Tyrosine protein kinase BTK, XLA

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Validation Data Gallery

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  WB analysis using 21581-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 21581-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21581-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21581-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 21581-1-AP

  IP Result of anti-BTK (IP:21581-1-AP, 4ug; Detection:21581-1-AP 1:300) with mouse spleen tissue lysate 4800ug.

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Tested Applications

• Positive WB detected in: Daudi cells, mouse spleen tissue, L02 cells, Ramos cells
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 21581-1-AP is BTK Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTK fusion protein Ag16133
• Full Name: Bruton agammaglobulinemia tyrosine kinase
• Calculated molecular weight: 659 aa, 76 kDa
• Observed molecular weight: 70-76 kDa
• GenBank accession number: BC109079
• Gene symbol: BTK
• Gene ID (NCBI): 695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.; Authors - Weijia Sun
human	WB	Int J Mol Med; Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.; Authors - Linlin Sheng