BTK Polyclonal antibody
BTK Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 21581-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, mouse spleen tissue, L02 cells, Ramos cells |
Positive IP detected in | mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 21581-1-AP is BTK Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BTK fusion protein Ag16133 |
Full Name | Bruton agammaglobulinemia tyrosine kinase |
Calculated molecular weight | 659 aa, 76 kDa |
Observed molecular weight | 70-76 kDa |
GenBank accession number | BC109079 |
Gene symbol | BTK |
Gene ID (NCBI) | 695 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for BTK antibody 21581-1-AP | Download protocol |
IP protocol for BTK antibody 21581-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction. | ||
Int J Mol Med Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. |