Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IF/ICC detected in | IMR-90 cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13330-1-AP targets BUB1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, carassius auratus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4138 Product name: Recombinant human BUB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 785-1083 aa of BC028201 Sequence: KLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRS Predict reactive species |
| Full Name | budding uninhibited by benzimidazoles 1 homolog (yeast) |
| Calculated Molecular Weight | 1085 aa, 122 kDa |
| Observed Molecular Weight | 45 kDa, 120 kDa |
| GenBank Accession Number | BC028201 |
| Gene Symbol | BUB1 |
| Gene ID (NCBI) | 699 |
| RRID | AB_2814999 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43683 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BUB1 antibody 13330-1-AP | Download protocol |
| WB protocol for BUB1 antibody 13330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BUB1 Polyclonal antibody (Cat# 13330-1-AP) has 16 citations. These appear in journals including Nucleic Acids Research, Journal of Experimental & Clinical Cancer Research, Frontiers in Immunology, Science Signaling, Cancer Gene Therapy, Breast Cancer Research, Frontiers in Pharmacology, Cancer Science, Scientific Reports, Molecular Biology Reports, BMC Gastroenterology, Gene, Thoracic Cancer, Journal of Thoracic Disease, and Clinical, Cosmetic and Investigational Dermatology. Research fields center on cancer biology—prostate, lung, ovarian, breast, endometrial, colorectal, and pancreatic cancers—along with spindle assembly checkpoint and mitosis regulation.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism. | ||
Front Immunol Exploring the carcinogenic potential of bisphenol A in lung adenocarcinoma: molecular mechanisms, key gene insights, and immune microenvironment impacts. | ||
Sci Signal Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas | ||
Breast Cancer Res Unraveling malignant phenotype of peritumoral tissue: transcriptomic insights into early-stage breast cancer |
