BUB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BUB1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13330-1-AP

Synonyms

Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A

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Validation Data Gallery

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  WB analysis of MKN-45 using 13330-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13330-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13330-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 13330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of IMR-90 cells using 13330-1-AP

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY.

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  IF Staining of MDCK using 13330-1-AP

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IF/ICC detected in: IMR-90 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

13330-1-AP targets BUB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human, carassius auratus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BUB1 fusion protein Ag4138
• Full Name: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Calculated Molecular Weight: 1085 aa, 122 kDa
• Observed Molecular Weight: 45 kDa, 120 kDa
• GenBank Accession Number: BC028201
• Gene Symbol: BUB1
• Gene ID (NCBI): 699
• RRID: AB_2814999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

Publications

Species	Application	Title
human		Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	WB	Sci Signal; Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas; Authors - Matthew J Knarr
human	IHC,IF	Cancer Sci; Stathmin guides personalized therapy in oral squamous cell carcinoma.; Authors - Wu-Tong Ju
Carassius auratus	WB	Front Bioeng Biotechnol; A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.; Authors - Wenting Xu
human	WB	J Exp Clin Cancer Res; Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.; Authors - Liang Chen
human	WB	Front Pharmacol; Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis; Authors - Xie Mengyan