BUB3 Polyclonal antibody

BUB3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 27073-1-AP

Synonyms

BUB3, BUB3L, hBUB3



Tested Applications

Positive WB detected inHEK-293T cells, C6 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27073-1-AP is BUB3 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BUB3 fusion protein Ag25608
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC005138
Gene symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BUB3 antibody 27073-1-APDownload protocol
IHC protocol for BUB3 antibody 27073-1-APDownload protocol
IF protocol for BUB3 antibody 27073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan