BXDC1 Polyclonal antibody

BXDC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17584-1-AP

Synonyms

RPF2, Brix domain-containing protein 1, brix domain containing 1, bA397G5.4

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Validation Data Gallery

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  WB analysis of HeLa using 17584-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17584-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17584-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17584-1-AP (BXDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BXDC1 antibody (17584-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HL-60 cells, HEK-293T cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17584-1-AP targets BXDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BXDC1 fusion protein Ag11268
• Full Name: brix domain containing 1
• Calculated Molecular Weight: 306 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC035314
• Gene Symbol: BXDC1
• Gene ID (NCBI): 84154
• RRID: AB_1939419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.