BXDC5 Polyclonal antibody

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  WB analysis using 33133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33133-1-AP (BXDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 33133-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BXDC5 antibody (33133-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BXDC5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Brix domain-containing protein 5, Ribosome biogenesis protein RPF1, Ribosome production factor 1, Ribosome Production Factor 1 Homolog, RPF1

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells, RKO cells, RT-4 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33133-1-AP targets BXDC5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37505

  Product name: Recombinant human BXDC5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC016051

  Sequence: MAKAGDKSGSSGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQLSTVIPNSHVYYRRGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPTEHIPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL

• Full Name: brix domain containing 5
• Calculated Molecular Weight: 349 aa, 40 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC016051
• Gene Symbol: BXDC5
• Gene ID (NCBI): 80135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H9Y2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BXDC5 (Brix domain-containing protein 5), also known as ribosome biogenesis protein RPF1, is a protein containing a Brix domain and is localized in the nucleolus. The Brix domain is a region that shares homology with the yeast protein Pitx1 (ribosome biogenesis protein BRX1). BXDC5 is a key factor in ribosome biogenesis, participating in the maturation of the 60S ribosomal subunit. This protein exerts its function by binding to specific rRNA structures and acts as a downstream effector molecule of the Ran signaling pathway, involved in ribosome biogenesis. In colorectal cancer (CRC), BXDC5 influences the occurrence and development of tumors by regulating ribosome biogenesis.