Bacteriophage T7 RNA polymerase ELISA Kit
Cat no : KE30008
Synonyms
T7 polymerase, T7 RNA polymerase
Validation Data Gallery
Product Information
KE30008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The bacteriophage T7 RNA polymerase ELISA kit is to be used to detect and quantify protein levels of endogenous bacteriophage T7 RNA polymerase. The assay recognizes bacteriophage T7 RNA polymerase. An antibody specific for bacteriophage T7 RNA polymerase has been pre-coated onto the microwells. The bacteriophage T7 RNA polymerase protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for bacteriophage T7 RNA polymerase is added to detect the captured bacteriophage T7 RNA polymerase protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Bacteriophage T7 RNA polymerase ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | T7 RNA polymerase |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.2-12.5 ng/mL |
| Reactivity | Bacteriophage T7 |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1261050 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| T7 RNA polymerase | 93% | 91%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.18 | 0.15 | 2.14 |
| 2 | 8 | 1.73 | 0.06 | 3.47 |
| 3 | 8 | 0.84 | 0.02 | 2.60 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.06 | 0.28 | 4.03 |
| 2 | 16 | 1.74 | 0.08 | 4.48 |
| 3 | 16 | 0.85 | 0.06 | 6.59 |
Background Information
T7 RNA polymerase is a DNA-dependent RNA polymerase from bacteriophage T7, known for its high specificity and processivity. T7 RNA polymerase requires DNA templates and magnesium ions (Mg2+) as cofactors together in the process of RNA synthesis. Bovine serum proteins and spermine have a promoting effect on T7 RNA polymerase. One of its differences from bacterial RNA polymerase is that T7 RNA polymerase is not inhibited by the antibiotic rifamycin. T7 RNA polymerase is widely used in biotechnology for mRNA synthesis, ribozyme production, and synthetic biology applications. Its single-subunit structure (~99 kDa) simplifies engineering for improved properties. Recent studies highlight its role in CRISPR-based diagnostics due to rapid RNA amplification.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | T7 polymerase, T7 RNA polymerase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.