Bcl-XL Recombinant antibody, PBS Only

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  WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  WB result of Bcl-XL antibody (83330-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bcl-XL transfected HeLa cells. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83330-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

• Featured Product
• KD/KO Validated

Bcl-XL Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83330-1-PBS

Clone No.1E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

BCL2L1, 1E8, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

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Product Information

83330-1-PBS targets Bcl-XL in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag25604
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: Bcl-XL
• Gene ID (NCBI): 598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q07817
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.