Mouse Bcl2 Recombinant antibody, PBS Only

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  WB analysis of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

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  WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82469-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

Bcl2 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 82469-6-PBS

Clone No.240571H5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Bcl-2, 240571H5, B cell leukemia/lymphoma 2, Bcl 2

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Product Information

82469-6-PBS targets Bcl2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24260
• Full Name: B-cell leukemia/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: NM-009741
• Gene Symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10417
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.