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Beta Catenin Recombinant monoclonal antibody (80508-1-RR)

Filter: AllIHCIF/ICC

IHC staining of human colon using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 80508-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80508-1-RR (B-catenin antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 80508-1-RR
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (80508-1-RR, Clone: 3M24 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Beta Catenin Recombinant monoclonal antibody

Name: Beta Catenin Recombinant monoclonal antibody
Brand: Proteintech
SKU: 80508-1-RR

Beta Catenin Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 80508-1-RR

Clone No.3M24

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human colon tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80508-1-RR targets Beta Catenin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight	781 aa, 86 kDa
GenBank Accession Number	BC058926
Gene Symbol	Beta Catenin
Gene ID (NCBI)	1499
ENSEMBL Gene ID	ENSG00000168036
RRID	AB_3743344
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35222
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)