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Anti-Human Beta Catenin Rabbit Recombinant Antibody, PBS Only (98860-3-PBS)

Anti-Human Beta Catenin Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human Beta Catenin Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98860-3-PBS-1MG

Beta Catenin Uni-rAb^® Recombinant Antibody for IHC, FC (Intra)

Cat No. 98860-3-PBS

Clone No.253094B8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra)

Synonyms

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllIHCFC (Intra)

IHC staining of human colon using 98860-3-RR (same clone as 98860-3-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98860-3-RR (1928-5/80 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 98860-3-RR (same clone as 98860-3-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98860-3-RR (1928-5/80 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of MCF-7 using 98860-3-RR (same clone as 98860-3-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
FC experiment of A431 using 98860-3-RR (same clone as 98860-3-PBS)
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
FC experiment of HeLa using 98860-3-RR (same clone as 98860-3-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human Beta Catenin Rabbit RecAb (98860-3-RR, Clone: 253094B8) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98860-3-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98860-3-PBS targets Beta Catenin in IHC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight	781 aa, 86 kDa
GenBank Accession Number	BC058926
Gene Symbol	Beta Catenin
Gene ID (NCBI)	1499
ENSEMBL Gene ID	ENSG00000168036
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35222
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)