Beta Galactosidase Monoclonal antibody, PBS Only

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  WB analysis using 66586-1-Ig (same clone as 66586-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66586-1-Ig (same clone as 66586-1-PBS)

  WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells. This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66586-1-Ig (same clone as 66586-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66586-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Beta Galactosidase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66586-1-PBS

Clone No.4F4F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GLB1, 4F4F4, Acid beta-galactosidase, Beta-galactosidase, EBP

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Product Information

66586-1-PBS targets Beta Galactosidase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8069

  Product name: Recombinant human Beta galactosidase protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 329-677 aa of BC007493

  Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

• Full Name: galactosidase, beta 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 64-66 kDa, 76-85 kDa
• GenBank Accession Number: BC007493
• Gene Symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• RRID: AB_2881946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P16278
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.