Bora Polyclonal antibody

• 

  WB analysis of K-562 using 26866-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26866-1-AP (Bora antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26866-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26866-1-AP (Bora antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Bora Polyclonal Antibody for WB, ELISA

Cat No. 26866-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BORA, C13orf34, HsBora, Protein aurora borealis

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26866-1-AP targets Bora in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19123

  Product name: Recombinant human C13orf34 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 213-559 aa of BC056876

  Sequence: PGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASAKKYSLGSITSPSPISSPTFSPIEFQIGETPLSEQRKFTVHSPDASSGTNSNGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYRTSVIQIPFTLETQGEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQNQSSASEKELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLPMTDFVSGIAFSIENSHMCMSPLAESSVIPCESSNIQMDSGYNTQNCGSNIMDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQCSSP

• Full Name: chromosome 13 open reading frame 34
• Calculated Molecular Weight: 559 aa, 61 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC056876
• Gene Symbol: Bora
• Gene ID (NCBI): 79866
• RRID: AB_2880662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6PGQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu