Broad range prestained protein marker
Molecular Weight
3-245 kDa
Detection Method
Colorimetric
Number of Markers
13
Stain Type
3 colors: Blue, Red, Green
Cat no : PL00002
"Broad range prestained protein marker" Comparison
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Product Information
The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.
Usage
The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.
Detection Method
Colorimetric
Molecular Weight
~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa
Number of Markers
13
Size Range
3 to 245
Stain Type
3 colors: Blue, Red, Green
Cited in Article as
PL00002, Broad range prestained protein marker, Proteintech, IL, USA
Documentation
| SDS |
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| Prestained_Potein Marker 3-245kDa SDS |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andrea (Verified Customer) (12-03-2022) | A good, strong signal is provided by loading a few microliters of the product.
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FH Dan (Verified Customer) (09-30-2022) | Low price but you get a very good quality of protein ladder!
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FH Mary (Verified Customer) (09-19-2022) | Worked okay. It's a decent protein marker.
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FH Cloure (Verified Customer) (08-25-2022) | Worked pretty decently
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FH Mickael (Verified Customer) (08-15-2022) | Worked like a charm!
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FH Maryna (Verified Customer) (08-13-2022) | Was very happy with the results, will ask my PI for to change
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FH Francisco (Verified Customer) (08-13-2022) | Performed very well in a pilot gel!
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FH Miguel (Verified Customer) (08-09-2022) | Very nice, will be switching when starting new project
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FH Miguel (Verified Customer) (08-05-2022) | It worked great.
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FH Duncan (Verified Customer) (10-20-2021) | Perfect for all my Western blotting
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FH Adrian (Verified Customer) (09-02-2021) | Protein ladder we obtained from colleagues from Biochemistry worked great and exhibited clear, distinguishable bands. Good overall quality for research on food allergenicity. Would highly recommend.
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FH Maria (Verified Customer) (08-23-2021) | Cell lysate 10ug labelled with No-stain (590 nm emission). 4-15% TGX gel. PL00002 5 uL on the left. Very good marker.
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FH Phoebe (Verified Customer) (03-10-2020) | This ladder worked brilliantly on my western. I have a large protein so need a broader-range ladder, for which I have previously been using PageRuler Plus. The new Proteintech ladder has been a very good replacement.
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