Broad Range Prestained Protein Marker
Number of Markers
3 colors: Blue, Red, Green
Cat no : PL00002
The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.
|Molecular Weight||~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa|
|Number of Markers||13|
|Size Range||3 to 245|
|Stain Type||3 colors: Blue, Red, Green|
|Contents||Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).|
|Storage||Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.
*This product may ship at ambient temperature. Upon receiving this product, immediately store product at the recommended temperatures.
|Instructions||The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.|
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