C-Peptide Recombinant monoclonal antibody

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• C-Peptide Recombinant monoclonal antibody (87566-3-RR)

C-Peptide Uni-rAb^® Recombinant Antibody for IHC, IF-P, ELISA

Cat No. 87566-3-RR Copied!

Clone No. 253514D7

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Synonyms

INS, insulin

• 

  IHC staining of human pancreas using 87566-3-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 87566-3-RR (C-Peptide antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 87566-3-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 87566-3-RR (C-Peptide antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human pancreas using 87566-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using C-Peptide antibody (87566-3-RR, Clone: 253514D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite® Plus 647 Somatostatin antibody (CL647-84351, Clone: 240816D9, Magenta), Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human pancreas using 87566-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using C-Peptide antibody (87566-3-RR, Clone: 253514D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite® Plus 647 Somatostatin antibody (CL647-84351, Clone: 240816D9, Magenta), Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human pancreas tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87566-3-RR targets C-Peptide in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: INS
• Calculated Molecular Weight: 110aa, 12 kDa
• GenBank Accession Number: NM_000207
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01308
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C‑peptide is a 31‑amino‑acid peptide that connects the A and B chains of proinsulin and is co‑secreted with insulin in equimolar amounts from pancreatic beta cells. With a longer plasma half‑life than insulin, it serves as a reliable biomarker of endogenous insulin secretion. Clinically, measuring C‑peptide is crucial for distinguishing type 1 from type 2 diabetes, diagnosing hypoglycemia, and assessing residual beta‑cell function. Emerging evidence also suggests that C‑peptide may exert its own biological effects on vascular and renal tissues.