C-Reactive Protein/CRP Polyclonal antibody

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  WB analysis using 31251-1-AP

  Rat liver tissue was subjected to SDS PAGE, followed by western blot with 31251-1-AP (C-Reactive Protein/CRP antibody) at a dilution of 1:3000 incubated at room temperature for 1.5 hours.

C-Reactive Protein/CRP Polyclonal Antibody for WB, ELISA

Cat No. 31251-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

PTX1, PTX 1, CRP, C-Reactive Protein

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Tested Applications

• Positive WB detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31251-1-AP targets C-Reactive Protein/CRP in WB, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: C-reactive protein, pentraxin-related
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: NM_017096.3
• Gene Symbol: Crp
• Gene ID (NCBI): 25419
• RRID: AB_3669919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48199
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation. This antibody is specific for rat Crp.