MCM-BP Polyclonal antibody

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  WB analysis of HeLa using 19573-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19573-1-AP (MCM-BP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19573-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19573-1-AP (MCM-BP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MCM-BP Polyclonal Antibody for WB, ELISA

Cat No. 19573-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C10orf119, MCM BP, MCMBP

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19573-1-AP targets MCM-BP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13750

  Product name: Recombinant human C10orf119 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 291-640 aa of BC004183

  Sequence: AEEQRVHSPPASLVPRIHVILAQKLQHINPLLPACLNKEESKTFVSSFMSELSPVRAELLGFLTHALLGDSLAAEYLILHLISTVYTRRDVLPLGKFTVNLSGCPRNSTFTEHLYRIIQHLVPASFRLQMTIENMNHLKFIPHKDYTANRLVSGLLQLPSNTSLVIDETLLEQGQLDTPGVHNVTALSNLITWQKVDYDFSYHQMEFPCNINVFITSEGRSLLPADCQIHLQPQLIPPNMEEYMNSLLSAVLPSVLNKFRIYLTLLRFLEYSISDEITKAVEDDFVEMRKNDPQSITADDLHQLLVVARCLSLSAGQTTLSRERWLRAKQLESLRRTRLQQQKCVNGNEL

• Full Name: chromosome 10 open reading frame 119
• Calculated Molecular Weight: 640 aa, 73 kDa
• Observed Molecular Weight: 73 kDa, 65 kDa
• GenBank Accession Number: BC004183
• Gene Symbol: MCM-BP
• Gene ID (NCBI): 79892
• RRID: AB_10734318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTE3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito