C10orf18 Polyclonal antibody

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  IHC staining of human breast cancer using 20420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20420-1-AP (C10orf18 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20420-1-AP (C10orf18 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C10orf18 Polyclonal Antibody for IHC, ELISA

Cat No. 20420-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

C10orf18, KIAA2006

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20420-1-AP targets C10orf18 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14240

  Product name: Recombinant human C10orf18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2081-2430 aa of BC001759

  Sequence: NSQRNHTVSFHLNKLKYNSTVKESRNDISLILNEYAEFNKVMKNSNQFIFQDKELNDVSGEATAQEMYLPFPGRSASYEDIIIDVCTNLHVKLRSVVKEACKSTFLFYLVETEDKSFFVRTKNLLRKGGHTEIEPQHFCQAFHRENDTLIIIIRNEDISSHLHQIPSLLKLKHFPSVIFAGVDSPGDVLDHTYQELFRAGGFVISDDKILEAVTLVQLKEIIKILEKLNGNGRWKWLLHYRENKKLKEDERVDSTAHKKNIMLKSFQSANIIELLHYHQCDSRSSTKAEILKCLLNLQIQHIDARFAVLLTDKPTIPREVFENNGILVTDVNNFIENIEKIAAPFRSSYW

• Full Name: chromosome 10 open reading frame 18
• Calculated Molecular Weight: 2430 aa, 269 kDa
• GenBank Accession Number: BC001759
• Gene Symbol: C10orf18
• Gene ID (NCBI): 54906
• RRID: AB_2878684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VWN6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.